FIELD: biotechnology, genetics and selection of plants. SUBSTANCE: morphogenic callus cultures obtained from the male fertile plants were treated with streptomycin aqueous solution for 24 h at streptomycin concentration 0.5-1.0 mg/ml. Plant-regenerants were obtained from treated callus and forms with the male sterility were detected. Method ensures to obtain the male sterile regenerants at high frequency (up to 100%) and sterility is inherited as cytoplasmic mutation. Invention can be used for preparing mutant cellular lines used in genetic and cellular engineering. EFFECT: improved method of preparing. 5 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PRODUCING FERTILE SORGHUM LINES THAT ARE REDUCING AGENTS FERTILITY FOR CMS TYPE 9 (E) | 2014 |
|
RU2599437C2 |
| METHOD OF REGENERATION OF CULTURED SORGHUM PLANT IN VITRO | 1999 |
|
RU2175189C2 |
| METHOD OF OBTAINING SORGHUM HYBRIDS F1 | 1999 |
|
RU2152713C1 |
| METHOD OF IN VITRO PLANT-REGENERANTS PREPARING | 1992 |
|
RU2027757C1 |
| METHOD FOR PREPARING COLZA (BRASSICA NAPUS L) FERTILITY RESTORER | 2007 |
|
RU2366705C2 |
| METHOD OF OBTAINING TRANSGENIC SORGHUM PLANTS | 2002 |
|
RU2229793C1 |
| METHOD OF OBTAINING FERTILITY RECOVERING AGENTS FOR STERILE CYTOPLASMS OF SORGHUM TYPES M-35-1A AND 9E | 2010 |
|
RU2545688C2 |
| DNA MARKER FOR SELECTION OF SORGHUM HYBRIDS BASED ON A1 TYPE CYTOPLASMATIC MALE STERILITY | 2021 |
|
RU2792135C1 |
| METHOD OF OBTAINING REGENERANTS OF FIBRE-FLAX, RESISTANT TO ANTHRACNOSE, BY METHODS IN VITRO | 2011 |
|
RU2478282C2 |
| METHOD OF PROPAGATION OF SORGHUM IN VITRO | 0 |
|
SU1107799A1 |
