FIELD: medicine.
SUBSTANCE: invention represents the method for preparing colza fertility restorer in vitro. The donor material is sampled, and central colza trusses are preconditioned with sterile cytoplasm and fertility restoration genes at temperature +4-6°C within a day. Unfertilised bud ovules of length 2-4 mm are introduced into the culture and cultivated in nutrient medium MS with kinetin 1 mg/l, naphthyl acetic acid 0.2 mg/l and 2.4-D 0.1 mg/l added. It is followed with stabilisation and microreproduction of haploid lines, diploidisation by 0.005% colchicin added to the nutrient medium. The established plants are relocated into the ground where they seed and thereafter sowed in field for growing the fertility restoring plants.
EFFECT: invention allows preparing a new raw material for CMS-based heterotic selection.
2 dwg, 2 tbl, 1 ex
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