FIELD: molecular biology, genetic engineering. SUBSTANCE: invention proposes plasmids used for replication in cells Escherichia coli and cyanobacterium. Plasmids have the following structural units; site of replication onset (oriA), multiple cloning site, region of replication onset (oriE) where a gene determining rop-function is removed. Plasmid pBAX18 has a size 6.9 thousand base pairs and plasmid pBAX20 - 5.8 thousand base pairs. The constructed plasmids ensure to enhance frequency of transformation of the strain Anacystis nidulans 6301. EFFECT: vector plasmid indicated above, enhanced effectiveness, simplified genetic procedures. 4 cl, 3 dwg, 1 tbl, 2 ex
| Title |
Year |
Author |
Number |
| VECTOR PLASMIDE DNA FOR CLONING FOREIGN GENES IN ESCHERICHIA COLI AND BACILLUS SPP GENES, AND METHOD OF ITS CONSTRUCTION |
0 |
- Shevchenko D.V.
- Dobritsa A.P.
|
SU1476896A1 |
| PLASMIDS FOR CHROMOSOME RECOMBINATION OF ESCHERICHIA COLI |
2001 |
- Takano Junichi
- Kino Kuniki
- Furukava Satoru
|
RU2314346C2 |
| RECOMBINANT PLASMID DNA pHIASP03, ENCODING HYBRID PROTEIN WITH HUMAN PROINSULIN Aspart, CELL Escherichia coli TRANSFORMED BY RECOMBINANT PLASMID DNA pHIAsp03, AND STRAIN OF BACTERIA Escherichia coli JM109/pHIAsp03 - PRODUCER OF HYBRID PROTEIN WITH HUMAN PROINSULIN Aspart |
2013 |
- Shmatchenko Vadim Vasil'Evich
- Sadgjan Armen Sergeevich
|
RU2514578C1 |
| RECOMBINANT PLASMID DNA PPINS07 ENCODING FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN AND STRAIN OF BACTERIUM ESCHERICHIA COLI - PRODUCER OF FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN |
1999 |
- Korobko V.G.
- Boldyreva E.F.
- Shingarova L.N.
- Vul'Fson A.N.
- Kostromina T.I.
- Miroshnikov A.I.
- Gavrikov V.G.
- Kalinin Ju.T.
- Stepanov A.V.
|
RU2144957C1 |
| RECOMBINANT PLASMID DNA pEFN877, DETERMINING EXPRESSION OF THE HYBRID POLYPEPTIDE WITH PROPERTIES OF 10-TH FIBRONECTIN DOMAIN ON THE SURFACE OF ESCHERICHIA COLI CELLS, AND STRAIN OF BACTERIA ESCHERICHIA coli BL21(DE3)pLysS/pEFN877 - PRODUCER OF HYBRID POLYPEPTIDE WITH PROPERTIES OF 10-TH FIBRONECTIN DOMAIN ON THE CELLS SURFACE |
2015 |
- Petrovskaya Lada Evgenevna
- Shingarova Lyudmila Nikolaevna
- Kryukova Elena Aleksandrovna
- Boldyreva Elena Filippovna
- Zlobinov Aleksandr Vladimirovich
- Dolgikh Dmitrij Aleksandrovich
- Kirpichnikov Mikhail Petrovich
|
RU2606014C1 |
| RECOMBINANT PLASMID DNA pFGM17 ENCODING POLYPEPTIDE OF HUMAN GRANULOCYTIC-MACROPHAGAL COLONY-STIMULATING FACTOR AND STRAIN OF BACTERIUM Escherichia coli BL21(DE3)/pFGM17 AS PRODUCER OF POLYPEPTIDE OF HUMAN GRANULOCYTIC-MACROPHAGAL COLONY-STIMULATING FACTOR |
2004 |
- Petrovskaja Lada Evgen'Evna
- Krjukova Elena Aleksandrovna
- Shingarova Ljudmila Nikolaevna
- Kirpichnikov Mikhail Petrovich
|
RU2271392C1 |
| RECOMBINANT PLASMID DNA PPINS16 ENCODING FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN, RECOMBINANT PLASMID DNA PPINS25 ENCODING FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN AND STRAIN OF BACTERIUM ESCHERICHIA COLI - PRODUCER OF FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN |
1999 |
- Korobko V.G.
- Boldyreva E.F.
- Shingarova L.N.
- Miroshnikov A.I.
- Gavrikov V.G.
- Stepanov A.V.
- Kalinin Ju.T.
|
RU2143493C1 |
| RECOMBINANT PLASMID DNA pHI03, CODING HYBRID PROTEIN WITH HUMAN PROINSULIN, CELL Escherichia coli, TRANSFORMED BY RECOMBINANT PLASMID DNA pHI03, AND STRAIN OF BACTERIA Escherichia coli JM109/pHI03-PRODUCENT OF HYBRID PROTEIN-PRECURSOR OF HUMAN INSULIN |
2013 |
- Shmatchenko Vadim Vasil'Evich
|
RU2515061C1 |
| RECOMBINANT PLASMID DNA pHILP07 ENCODING HYBRID PROTEIN WITH HUMAN PROINSULIN Lispro, CELL Escherichia coli TRANSFORMED BY RECOMBINANT PLASMID DNA pHILP07, AND STRAIN OF BACTERIA Escherichia coli JM109/pHILP07 - PRODUCER OF HYBRID PROTEIN WITH HUMAN PROINSULIN Lispro |
2013 |
- Shmatchenko Vadim Vasil'Evich
|
RU2514480C1 |
| VECTOR PIL207 PROVIDING REGULATED EXPRESSION IN ESCHERICHIA COLI, AND A METHOD OF ITS CONSTRUCTION |
1986 |
- Solonin A.S.
- Trojanovskij B.M.
- Ksenzenko V.N.
- Baev A.A.
|
SU1405313A1 |
