FIELD: genetic engineering. SUBSTANCE: invention reports the construction of recombinant plasmid DNA pHIN representing Eco RI-Xho I-fragment of bifunctional bacterial-yeast vector pPIC19 ligated with encoding region of human gene alpha-interferon-16 but with deletion in region encoding signaling peptide. As result of transformation of the strain Pichia pastoris PS 99 with plasmid pHIN the recombinant strain Pichia pastoris PS 104 (pHIN) is obtained that provides both effective synthesis of human alpha-interferon- 16 (15 mg per 1 l of culture) and secretion of the end product by cells. Invention can be used in microbiological and medicinal industry. EFFECT: valuable properties of strain, increased yield of interferon. 3 cl, 2 dwg
| Title |
Year |
Author |
Number |
| YEAST STRAIN PICHIA PASTORIS AS PRODUCER OF HUMAN FIBROBLAST INTERFERON, RECOMBINANT PLASMID DNA PHIS AND METHOD OF ITS CONSTRUCTION |
2000 |
- Padkina M.V.
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- Smirnov M.N.
|
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2002 |
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|
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2000 |
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|
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2006 |
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|
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2011 |
|
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|
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1998 |
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|
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|
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2020 |
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|
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|
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