METHOD OF SYNTHESIS OF BIOTIN-LABELED OLIGODEOXYRIBONUCLEOTIDES Russian patent published in 1997 - IPC

Abstract RU 2083584 C1

FIELD: biotechnology, molecular biology, medicine. SUBSTANCE: method involves interaction of biotin-containing reagent with spacer amino-group that is incorporated preliminary in oligonucleotide. Biotin-containing reagent: 4-sulfo-3-nitrophenyl or sulfotetrafluorophenyl ether of biotin. Process is carried out in an aqueous solution. Method is used for identification of the examined biomaterial, in part, at nucleic acid hybridization analysis including viral nucleic acids using the detector avidin-biotin (streptavidin-biotin) system. Method provides preparing biotin-labeled oligonucleotides at preparative amounts. EFFECT: high yield, decreased time of synthesis.

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RU 2 083 584 C1

Authors

Godovikova T.S.

Orlova T.N.

Abramova T.V.

Kulikova V.F.

Zalite I.K.

Medvedkin V.N.

Dates

1997-07-10—Published

1994-02-08—Filed