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2 049 821

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C1

(51)

IPC

(21) (22)

Application

92001266/13, 1992-10-20

(22)

Actual filing date

1992-10-20

(45)

Published

1995-12-10

(72)

Inventor

Adarichev V.A.Dymshits G.M.Kalachikov S.M.

(73)

Holder

Institut Tsitologii I Genetiki So Ran

METHOD OF PREPARING NON-RADIOACTIVE LABELLED PROBE FOR DETERMINATION OF NUCLEOTIDE SERIES Russian patent published in 1995 - IPC

Abstract RU 2049821 C1

FIELD: biotechnology. SUBSTANCE: origin nucleic acid is modified by reamination at pH 3.2-4.5 and temperature 60-90 C within 1-12 min. Reagent having formula R1-S-R2 where R1 is derivative of hydroxyl amine which reacts with cytosine of nucleic acid, R2 is chemical group which reacts with activated derivative being used as labelling agent is used as reamination agent. EFFECT: improves efficiency of method. 2 cl, 2 dwg

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RU 2 049 821 C1

Authors

Adarichev V.A.

Dymshits G.M.

Kalachikov S.M.

Dates

1995-12-10—Published

1992-10-20—Filed

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