FIELD: biotechnology, medicine. SUBSTANCE: method involves construction of recombinant plasmid pSStgXS-TARG containing DNA sequence that encodes ovine β-lactoglobulin fused with factor IX or with a1-antitrypsin. From the obtained plasmid fragment involving at least regulatory and secretory signal sequences of β-lactoglobulin fused with the sequence of gene encoding the end protein is isolated. Fragment is incorporated in the fertilized sheep ovicell or embryo followed by transplantation of ovicell or embryo in recipient ewes and selection of transgenic animals. EFFECT: improved method of producing. 9 dwg, 3 tbl
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