DNA MOLECULE, EXPRESSION VECTOR Russian patent published in 1999 - IPC

FIELD: genetic engineering, molecular biology. SUBSTANCE: invention relates to DNA encoding BSSL/CEL and expression vectors. DNA has a nucleotide sequence SEQID No 1. Vector has 2.7 thousand base pairs. NotI-NatI is a fragment of plasmid pUC19 which involves gene (amp) determining resistance to ampicillin, 5'-flanking and 5'-nontranslated regions WAP of mouse on fragment NotI-KpnI (size is 4.1 thousand base pairs), human structural gene BSSL on KpnI-SalI-fragment (size is 9.9 thousand base pairs), 3'-flanking region and a structural mouse gene WAP on SalI-NotI-fragment (size is 4.3 thousand base pairs). The use of this DNA ensures to obtain recombinant human LSBAS/CEL that can be used as a component for children nutrition. EFFECT: DNA and vector indicated above, improved method of preparing. 3 cl, 3 tbl, 12 dwg, 4 ex