FIELD: biotechnologies.
SUBSTANCE: invention relates to field of biotechnology and genetic engineering. Method includes cloning genetic structure coding hGH gene, and, correspondingly, beta-casein promoter into expression vector. Further, transfection into female somatic cells, especially fibroblasts, is performed, and nuclei are transferred into enucleated bull oocytes, which results in formation of transgenic embryos. Also claimed are methods of obtained recombinant growth hormone purification from milk of transgenic mammals. Thus, transgenic embryos give rise to transgenic cattle that produces human growth hormone in its milk in enormous quantity, and from which hormone was fully isolated and analysed for performing all necessary conditions for pure biopharmaceutical product manufacturing.
EFFECT: invention can be used in medicine and veterinary.
48 cl, 16 dwg, 3 tbl, 9 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF CREATING TRANSGENIC MAMMALS WHICH PRODUCE EXOGENOUS PROTEINS IN MILK, AND TRANSGENIC MAMMALS CREATED USING SAID METHOD | 2004 |
|
RU2390562C2 |
| TRANSGENE FOR PREPARING THE RECOMBINANT POLYPEPTIDE IN TRANSGENIC COW MILK, A METHOD OF OBTAINING THE TRANSGENIC COW (VARIANTS), MILK FROM TRANSGENIC COW, FOOD COMPOSITION | 1990 |
|
RU2095414C1 |
| PROTEIN PRODUCTION | 2005 |
|
RU2429243C2 |
| METHOD OF OBTAINING RECOMBINANT PROTEINS IN MAMMARY GLAND OF NON-TRANSGENIC MAMMALS | 2003 |
|
RU2345088C2 |
| PRODUCTION OF BIOLOGICALLY ACTIVE PROTEINS | 2007 |
|
RU2441911C2 |
| TRANSGENIC HOOFED ANIMALS, HAVING LOWER ACTIVITY OF PRION PROTEIN, AND THEIR APPLICATIONS | 2003 |
|
RU2384059C2 |
| VARIANTS OF LIPASE STIMULATING BY BILE SALTS, DNA ENCODING THEREOF AND TRANSGENE MAMMALS NO BELONGING TO HUMANS | 1994 |
|
RU2219239C2 |
| PROTEIN RECOVERY AND PURIFICATION | 2005 |
|
RU2420585C2 |
| DNA MOLECULE, EXPRESSION VECTOR | 1993 |
|
RU2128708C1 |
| METHOD FOR OBTAINING ANIMAL EMBRYOS AND METHOD FOR RAISING ANIMALS OUT OF EMBRYOS | 1998 |
|
RU2216592C2 |
