FIELD: molecular biology, genetic engineering. SUBSTANCE: human granulocyte colony-stimulating factor is prepared by expression of recombinant proteins in mammary gland of transgenic animals. Milk of transgenic animals is used for preparing human granulocyte colony-stimulating factor. Original genic structures of human granulocyte colony-stimulating factor based on available genomic DNA copy and regulatory genes of milk proteins providing effective secretion of human granulocyte colony-stimulating factor with milk of transgenic animals have been developed. Invention can be used in immunology. EFFECT: improved method of transgenic animal producing. 3 cl, 3 dwg, 2 ex
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