FIELD: biotechnology, genetic engineering, veterinary science, molecular biology.
SUBSTANCE: invention can be used in microbiological industry and agriculture. Plasmid DNA pBIG providing the biosynthesis of bovine intracellular gamma-interferon is constructed by excision of yeast Saccharomyces cerevisiae gene MFα prepro-region from bacterial-yeast vector pPIC9 with restriction endonucleases Bam HI and Xho I. The yeast strain Pichia pastoris PS105(pBIG) as a producer of bovine intracellular immune interferon is obtained and deposited in All-Russian collection of industrial microorganisms at number VKPM-Y-2990. Application of invention provides preparing the bovine intracellular gamma-interferon with the enhanced yield.
EFFECT: improved preparing method, valuable biological properties of strain.
4 cl
| Title |
Year |
Author |
Number |
| PICHIA PASTORIS YEAST STRAIN - PRODUCER OF CHICKEN INTERFERON-GAMMA, RECOMBINANT PLASMID DNA WHICH FACILITATES BIOSYNTHESIS CHICKEN INTERFERON-GAMMA, AND CONSTRUCTION METHOD THEREOF |
2006 |
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- Gradoboeva Anastasija Evgen'Evna
- Sambuk Elena Viktorovna
- Smirnov Mikhail Nikolaevich
|
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2006 |
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- Padkina Marina Vladimirovna
- Sambuk Elena Viktorovna
- Smirnov Mikhail Nikolaevich
|
RU2315806C1 |
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2000 |
- Padkina M.V.
- Parfenova L.V.
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|
RU2180687C1 |
| STRAIN OF YEAST PICHIA PASTORIS AS PRODUCER OF LEUKOCYTE HUMAN INTERFERON-16, RECOMBINANT PLASMID DNA PHIN AND METHOD FOR ITS CONSTRUCTION |
2002 |
- Padkina M.V.
- Parfenova L.V.
- Sambuk E.V.
- Smirnov M.N.
|
RU2203950C1 |
| RECOMBINANT PLASMID, METHOD FOR ITS CONSTRUCTION AND YEAST STRAIN KOMAGATAELLA PASTORIS - PRODUCER OF IMMUNE INTERFERON GAMMA OF A DOG |
2020 |
- Muzaev Dmitrij Mikhajlovich
- Padkina Marina Vladimirovna
|
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| RECOMBINANT PLAZMID DNA pART27INT6 AND METHOD OF OBTAINING ON ITS BASIS OF INBRED LINE OF TOBACCO PLANTS SYNTHESISING INTRACELLULAR GAMMA INTERFERON OF BULL |
2014 |
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- Padkina Marina Vladimirovna
- Savel'Eva Natal'Ja Vladimirovna
- Emel'Janov Vladislav Vladimirovich
- Burlakovskj Mikhail Sergeevich
- Zobnina Anastasija Evgen'Evna
- Tkachenko Aleksandr Anatol'Evich
|
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| RECOMBINANT PLASMID DNA PHBS PROVIDING BIOSYNTHESIS OF SURFACE ANTIGEN OF HEPATITIS B VIRUS, METHOD OF CONSTRUCTION OF PLASMID, YEAST STRAIN PICHIA PASTORIS PS103 (PHBS) AS PRODUCER OF INDICATED ANTIGEN |
2000 |
- Padkina M.V.
- Parfenova L.V.
- Sambuk E.V.
- Smirnov M.N.
|
RU2201452C2 |
| RECOMBINANT PLASMID DNA DETERMINING SYNTHESIS OF HUMAN FIBROBLAST INTERFERON IN YEAST CELLS, METHOD OF ITS CONSTRUCTION AND YEAST STRAIN SACCHAROMYCES CEREVISIAE AS PRODUCER OF HUMAN FIBROBLAST INTERFERON |
1998 |
- Padkina M.V.
- Parfenova L.V.
- Sambuk E.V.
- Smirnov M.N.
|
RU2180003C2 |
| STRAIN YEAST PICHIA PASTORIS PS107(pPIC9HAbIL-2) AS PRODUCER OF HYBRID PROTEIN CONSISTING OF HUMAN PLASMA BLOOD ALBUMIN AND HUMAN INTERLEUKIN-2, RECOMBINANT PLASMID pPIC9HAbIL-2 AND METHOD FOR ITS CONSTRUCTING |
2006 |
- Padkina Marina Vladimirovna
- Zinov'Eva Julija Grigor'Evna
- Sambuk Elena Viktorovna
- Smirnov Mikhail Nikolaevich
|
RU2315105C1 |
| RECOMBINANT PLASMID DNA pPDGFB ENCODING A POLYPEPTIDE WITH HUMAN PLATELET-BB GROWTH FACTOR HUMAN PROPERTIES, AND A RECOMBINANT STRAIN OF METHYLOTROPHIC YEAST PICHIA PASTORIS, A PRODUCER OF A POLYPEPTIDE WITH HUMAN PLATELET-BB GROWTH FACTOR HUMAN PROPERTIES |
2017 |
- Gerasimov Andrej Sergeevich
- Litvinets Sergej Gennadevich
|
RU2668828C1 |
