FIELD: chemistry; biochemistry.
SUBSTANCE: invention relates to biotechnology and is a Pachia pastoris PS 108 (pChIG) yeast strain, which is a producer of chicken interferon-gamma transformed by plasmid pChIG. Such plasmid pChIG, which facilitates biosynthesis of chicken interferon-gamma, has size of 8.19 thousand base pairs and consists of the following elements: - EcoRI-BamHI - a fragment of plasmid DNA of bifunctional bacterial-yeast vector pPIC9 with size of 7.75 thousand base pairs, - Bglll-EcoRI - a fragment with size of 0.44 thousand base pairs, which contains the coding part of the chicken interferon-gamma gene except the signal peptide coding region. The chicken interferon-gamma gene is obtained through a reverse polymerase chain reaction, whose matrix is RNA obtained from chicken peripheral blood mononuclear cells, induced by human recombinant interleukin-2 Roncoleikin ®.
EFFECT: invention enables obtaining chicken interferon-gamma with high efficiency.
3 cl, 2 dwg, 3 ex
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Year |
Author |
Number |
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