FIELD: biotechnology, genetic engineering. SUBSTANCE: mammalian cells culturing in vitro were pressed by cover glass perpendicularly to monolayer surface in PBS-buffer containing exogenous DNA at concentration 10-40 mcg/ml. Glass is removed and cells were incubated in the same solution at 37 C for 40-60 min. Method can be used for study of genetic members (promoters, enhancers) taking part in gene expression regulation, tissue specificity and related branches. Invention can be used in genetic engineering studies, agriculture, biotechnology, medicine, veterinary science. EFFECT: improved method of DNA incorporation, simplified method. 2 tbl
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Authors
Dates
1997-10-27—Published
1993-07-26—Filed