FIELD: medicine.
SUBSTANCE: method provides transplantation of embryos all cells of which contain the additional genetic information to a recipient female. The introduction of the additional genetic information in a single-cell embryo is performed in a medium containing 150 mM NaCl, 2 mM HEPES and nucleic acid in the concentration 5 ng/mcl by generating two electric pulse trains of the filed intensity 100-200 V/cm of the duration 750 ms every 30 s.
EFFECT: method allows substantially increasing a number of produced transgene mice and reducing process length.
2 cl, 1 dwg, 2 ex
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