FIELD: biotechnology, genetic engineering. SUBSTANCE: invention relates to a method of preparing the strain of bacterium assigned for stable storage of plasmid having DNA fragment that provides the betaine utilization from substrate. Method involves carrying out the mutation in chromosomal gene of the strain of bacterium Rhizobium/Agrobacterium that forms deficient strain with respect to betaine utilization. Method involves construction of recombinant plasmid DNA pL 032 containing DNA fragment providing betaine utilization from substrate that has the indicated restriction map. Then recombinant plasmid DNA is transferred in the strain containing mutation followed by culturing the transformed strain of bacterium Rhizobium/Agrobacterium DSM N 6712. Recombinant plasmid DNA: pL 032. DNA fragment of size 3 kb containing the indicated restriction map. EFFECT: improved method of preparing. 6 cl, 1 tbl, 2 dwg
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Year |
Author |
Number |
| GENES OF METABOLISM OF BUTYROBETAINE/CROTONOBETAINE - L- -CARNITINE AND THEIR USE FOR MICROBIOLOGICAL SYNTHESIS OF L-CARNITINE |
1994 |
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- Jozef Verlen
|
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1992 |
- Tomas Tsimmermann
- Karen Robins
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- Ehlizabet Belen
|
RU2126450C1 |
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1991 |
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|
RU2088667C1 |
| DNA FRAGMENT (VARIANTS), FUSED DNA FRAGMENT FOR EXPRESSION IN PLANTS AND METHOD OF PRODUCING TRANSGENIC PLANT AND ITS OFFSPRINGS |
1989 |
- Dzhon Rajlz
- Alise Montoja
- Khristian Garmz
- Dzhon D'Jusing
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- Fred Mejns
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1999 |
- Korobko V.G.
- Boldyreva E.F.
- Shingarova L.N.
- Miroshnikov A.I.
- Gavrikov V.G.
- Stepanov A.V.
- Kalinin Ju.T.
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| RECOMBINANT PLASMIDE DNA PPR-TGATG-HIL-Iβ-TSR WHICH PROVIDES SYNTHESIS OF RECOMBINANT INTERLEIKIN-1 AND STRAIN BSCHERICHIA COLI BEING PRODUCER OF RECOMBINANT HUMAN INTERLEIKIN-1 b |
1992 |
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- Kozlov A.P.
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- Debabov V.G.
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| RECOMBINANT DNA FRAGMENT THAT CODES SPECIFIC NITRILASE DETERMINING PLANT RESISTANCE TO HERBICIDES BASED UPON 3.5-DIHALOID-4-OXYBENZONITRILE; EXPRESSION VECTOR FOR RECOMBINANT DNA FRAGMENT (VARIANTS) |
1989 |
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RU2106410C1 |
| VIRAL GENOME FRAGMENT OF CHICKEN K STRAIN VARIOLA WHICH CONTAINS THYMIDINE KINASE GENE, pTK1FPV AND pTK2FPV RECOMBINANT PLASMID DNAs WHICH CONTAIN THIS SEQUENCE, AND pINTFPV1 RECOMBINANT PLASMID DNA WHICH IS ABLE TO INTEGRATE INTO VIRAL GENOME OF CHICKEN VARIOLA |
1999 |
- Susloparov M.A.
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- Bakhtina M.M.
- Susloparov I.M.
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- Lokteva L.A.
|
RU2180352C2 |
| RECOMBINANT PLASMID DNA PPINS07 ENCODING FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN AND STRAIN OF BACTERIUM ESCHERICHIA COLI - PRODUCER OF FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN |
1999 |
- Korobko V.G.
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- Kostromina T.I.
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|
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1992 |
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RU2085586C1 |
