FIELD: genetic engineering, microbiology. SUBSTANCE: DNA fragment is taken from Eco RI-fragment (size is 10.6 thousand base pairs) which comprises genes encoding enzymes of L-carnitine biosynthesis in metabolism of gamma-butyrobetaine/crotonobetaine or variants of these genes. Genes present as a single transcription unit and located at the following order: bco C encoding γ-butyrobetaine-CoA dehydrogenase, bco A/B encoding γ-butyrobetaine/crotono-betaine-CoA synthetase and bco D encoding crotonobetaine-CoA hydrolase. Fragment can comprise the gene bco T encoding the potential transport protein. Expression vectors are p VK 100q, p VK 101, pAZ 101, pAZ 7, pAZ 7::beu, pL 041. Recombinant strains Rhizobium/Agrobacterium HK 1349 transformed with the corresponding expression vectors are producers of L-carnitine. EFFECT: increased yield of L-carnitine. 16 cl, 7 dwg, 1 tbl, 8 ex
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