FIELD: biotechnology. SUBSTANCE: method involves submerged culturing the microbe cells sowing material on liquid nutrient medium at shaking and aeration and separation of biomass by sedimentation. Nutrient medium used: a mixture consisting of cultural fluid after biomass sedimentation depleted from the previous culturing stage, and/or cultural fluid after plague vaccine rejection by technological parameters with part of fresh nutrient medium taken at amount 15-20%, not less, of the parent medium volume. EFFECT: improved method of biomass preparing. 4 tbl
Authors
Dates
1998-01-20—Published
1994-07-19—Filed