METHOD OF STEVIOSIDE PREPARING Russian patent published in 1998 - IPC

FIELD: biotechnology, pharmaceutical technology. SUBSTANCE: invention relates to methods of preparing stevioside from leaves of plant Stevia rebaudiana B. Method involves double an aqueous extraction of milled dried leaves of plant at 50-60 C, extract filtration and purification of extract by column chromatography on inorganic sorbent ((Al₂O₃)) followed by concentrating and drying. Double an aqueous extraction promotes more complete extraction of diterpene glycosides from raw. EFFECT: simplified method, decreased cost of technological process.