FIELD: food industry.
SUBSTANCE: present invention relates to a method of producing a combination of steviol glycosides used as sweeteners in food industry. Disclosed is a method of producing a combination of two or more steviol glycosides, involving extraction of stevia leaves with hot water, purification of the total extract and separation of a combination of two or more steviol glycosides by continuous chromatography, where continuous chromatography is carried out using gradient elution with two or more solvents, and a temperature gradient, where two or more solvents contain water, and where a combination of two or more steviol glycosides contains less than 25 % by weight of rebaudioside A.
EFFECT: disclosed is a novel effective complex separation method which enables to separate a combination of two or more steviol glycosides from stevia extract.
16 cl, 7 dwg, 9 tbl, 4 ex
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Authors
Dates
2019-05-22—Published
2015-02-18—Filed