FIELD: medicine. SUBSTANCE: method involves using minimum quantity of blood serum or homogenate (0.2-0.5 ml). Thiobarbituric acid is solved and sample is incubated in presence of triton X-100. The mixture is stirred under constant frequency of oscillations (120 swingings per 1 min). The reaction is stopped by applying dihydroquercetin. Trilon B and mixture of ethanol and chloroform are added in 7:3 proportion before measuring optic density of the sample. EFFECT: high accuracy and sensitivity of method.
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Authors
Dates
1998-05-27—Published
1995-09-08—Filed