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2 158 303

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IPC

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Application

98118945/13, 1998-10-19

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Start date

1998-10-19

(22)

Actual filing date

1998-10-19

(45)

Published

2000-10-27

(72)

Inventor

Lebedev L.R.Kan'Shina A.V.Litovchenko L.L.Krivopalova G.N.Masycheva V.I.Pustoshilova N.M.

(73)

Holder

Gosudarstvennyj Nauchnyj Tsentr Virusologii I Biotekhnologii

METHOD OF PRODUCING RECOMBINANT STRAINS OF BACTERIUM ESCHERICHIA COLI BIOMASS ENRICHED WITH POLYPEPTIDES WITH HUMAN CYTOKINE PROPERTIES Russian patent published in 2000 - IPC

Abstract RU 2158303 C2

FIELD: biotechnology, microbiology. SUBSTANCE: invention relates to biosynthesis of human cytokines (tumor necrosis alpha- and beta-factor, granulocyte and granulocyte-macrophage colony-stimulating factors) by recombinant strains of E. coli SG20050/pTNF311Δ, E. coli SG20050pLT21, E. coli SG20050/pGGG8 and E. coli SG20050/p280GM. For preparing inoculum with high content of plasmid-containing cells inoculate is treated with ampicillin in concentration 400-500 mcg/ml for 2-3 h. Ampicillin in concentration 100 mcg/ml is added to medium in the process of fermentation. Ampicillin is added in each 1-2 h of culture growth. EFFECT: decreased segregation nonstability of recombinant strains, increased content of end proteins in biomasses. 6 cl, 1 dwg, 6 tbl, 5 ex

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RU 2 158 303 C2

Authors

Lebedev L.R.

Kan'Shina A.V.

Litovchenko L.L.

Krivopalova G.N.

Masycheva V.I.

Pustoshilova N.M.

Dates

2000-10-27—Published

1998-10-19—Filed