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2 326 169

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IPC

C12N15/27(2006-01-01)

C12N15/63(2006-01-01)

C12N1/21(2006-01-01)

C12R1/19(2006-01-01)

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Application

2006137143/13, 2006-10-20

(24)

Start date

2006-10-20

(22)

Actual filing date

2006-10-20

(45)

Published

2008-06-10

(72)

Inventor

Shingarova Ljudmila NikolaevnaBoldyreva Elena FilippovnaPetrovskaja Lada Evgen'EvnaPustoshilova Nina MikhajlovnaGoncharova Ol'Ga VladimirovnaChugunova Nadezhda MuldabekovnaChugunov Aleksandr MikhajlovichEfanov Jurij Georgievich

(73)

Holder

Zakrytoe Aktsionernoe Obshchestvo

RECOMBINANT PLASMID DNA pA3GF CODING POLYPEPTIDE OF HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR, AND Escherichia BACTERIA RACE coli-PRODUCER OF HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR POLYPEPTIDE Russian patent published in 2008 - IPC C12N15/27 C12N15/63 C12N1/21 C12R1/19

Abstract RU 2326169 C1

FIELD: gene engineering.

SUBSTANCE: invention can be used for production of recombinant polypeptide of human granulocyte colony-stimulating factor. Recombinant plasmid DNA is constructed in vitro. It includes synthetic gene of human granulocyte colony-stimulating factor, strong constitutive promoter A3 from the early stage of bacteriophage T7 and synthetic section - translation enhancer (TREN) of gene 10 in bacteriophage T7. This DNA in combination with high copy number of plasmid and optimisation of cultivation conditions ensures constitutive biosynthesis of target protein in transformed by this DNA race of Escherichia coli SGK25/pA3GF with high yield.

EFFECT: constitutive biosynthesis of target protein in cells; high yield.

2 cl, 4 dwg, 7 ex

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RU 2 326 169 C1

Authors

Shingarova Ljudmila Nikolaevna

Boldyreva Elena Filippovna

Petrovskaja Lada Evgen'Evna

Pustoshilova Nina Mikhajlovna

Goncharova Ol'Ga Vladimirovna

Chugunova Nadezhda Muldabekovna

Chugunov Aleksandr Mikhajlovich

Efanov Jurij Georgievich

Dates

2008-06-10—Published

2006-10-20—Filed