FIELD: medicine, veterinary science. SUBSTANCE: method involves preparing samples from conjunctiva and nasal mucosa rinses containing carnivorous plague virus antigen treated with a mixture chloroform-alcohol at ratio 3:1 as an extractant. Then specific binding antibodies to virus to be tested and the second antibodies - protein A conjugate with horse radish peroxidase are prepared. Then antigen is attached to nitrocellulose backing and the latter with antigen is treated with a mixture of the first and the second antibodies prepared at ratio 20:1, not less, in protein-buffer solution. Obtained immunochemical complex is treated with chromogen on backing. Method can be used for laboratory serological diagnosis of viral diseases, in part, carnivorous plague virus. EFFECT: improved method of analysis.
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