RECOMBINANT PLASMID ENCODING FUSED PROTEIN-PRECURSOR OF HUMAN INSULIN (VARIANTS), STRAIN OF BACTERIUM ESCHERICHIA COLI - PRODUCER OF FUSED PROTEIN-PRECURSOR OF HUMAN INSULIN (VARIANTS), METHOD OF HUMAN INSULIN PREPARING Russian patent published in 2000 - IPC

FIELD: molecular biology, biotechnology, genetic engineering. SUBSTANCE: invention describes the novel developed plasmids expressing a fused protein where amino acid sequences of lider peptide that is JgG-binding domain of protein A and human proinsulin are bound by arginine residue (plasmid pRproins) or by lysine residue (plasmid pRproins). Human insulin is obtained by the method involving culturing the strain E. coli JM-109-pRproins carrying plasmid pRproins or the strain E. coli JM-109-pRproins carrying plasmid pRproins, isolation of bodies inclusion, solubilization, sulfitolysis and renaturation of fused protein followed by purification of fused protein by ion-exchange chromatorgaphy method, its cleavage with trypsin-like and carboxypeptidase B-like enzymes and isolation of insulin. EFFECT: simplified technological process, increased yield of human insulin. 6 cl, 4 dwg, 7 ex