RECOMBINANT ANTIBODY, POLYNUCLEOTIDE (VARIANTS), METHOD OF PREPARING HEAVY AND/OR LIGHT CHAINS OF RECOMBINANT ANTIBODY (VARIANTS) Russian patent published in 1999 - IPC

FIELD: molecular biology, biochemistry, immunology. SUBSTANCE: recombinant antibody reacts specifically with protein p55 of Tac-receptor of human interleukin-2 (IL-2). The indicated antibody inhibits binding of IL-2 with IL-2-receptor on human T-cells and shows affinity to IL-2 receptor above 10⁸M^-1. In consists of light and heavy chains and has sites that determine complementarity (CDR_s). Invention proposes also polynucleotides containing a sequence encoding carcass sites and sites determining complementarity of binding with antigen (CDR_s). Also, invention proposes methods of preparing heavy and/or light chains of recombinant antibody. Invention can be used for preparing recombinant DNA and nonimmunogenic monoclonal antibodies for preparing new therapeutic agents. EFFECT: improved method of preparing. 11 cl, 10 dwg, 1 tbl, 5 ex