RECOMBINANT VECTOR USABLE IN SYNTHESIZING PHYSIOLOGICALLY ACTIVE SUBSTANCE CONTAINING PROMOTOR SITE OF β-CASEIN GENE OF KOREAN LOCAL GOATS AND METHOD FOR PERFORMING THE PHYSIOLOGICALLY ACTIVE SUBSTANCE Russian patent published in 2002 - IPC

FIELD: medicine; genetic engineering. SUBSTANCE: method involves constructing recombinant vector pGbc (KCTC 8790P) containing promotor site of β-casein gene of Korean local goats on the basis of pBluescript II SK. Recombinant vector pGbc_L (KCTC 8791P) containing promoter site of β-casein gene of Korean local goats on the base of pRc/RSV. Structural gene is cloned into the built vector coding a physiologically active substance. The physiologically active substance is produced by culturing mammalian cells transfected by vector built on pGbc_L or pGbc_S basis and by creating transgenetic animal by means of vector produced on the basis of pGbc. EFFECT: enhanced effectiveness in producing physiologically active substances in mammalian expression system. 9 cl, 16 dwg