MEDIUM FOR RICE PROTOPLAST FUSING Russian patent published in 1999 - IPC

Abstract RU 2138552 C1

FIELD: biotechnology, physiology, plant tissue cultures. SUBSTANCE: in medium for fusion of rice protoplasts dimethylsulfoxide is added additionally at the following ratio of components, weight %: CaCl₂•2H₂O 0.0478-0.0615; KH₂PO₄ 0.054-0.0695; KNO₃ 0.0956-1.231; (NH₄)₂SO₄ 0.0427-0.0549; MgSO₄•7H₂O 0.0796-0.103; MnSO₄•H₂O 0.0032-0.0041; ZnSO₄•7H₂O 0.00064-0.00082; MnSO₄•4H₂O 0.0042-0.0054; H₃BO₃ 0.0009-0.0012; KJ 0.00024-0.0003; CuSO₄•5H₂O 0.000007-0.000009; Na₂MoO₄•2H₂O 0.00007-0.00009; CoCl₂•6H₂O 0.000007-0.0000009; Fe₂SO₄•7H₂O 0.0089-0.0111; HO(CH₂H₄O)₈₈H 34.97028- -40.705808; C₆H₁₄O₆ 22.310-28.716; NaOH 6.374-8.205; NH₂CH₂COOH 6.374- -7.705; C₂H₆SO 15.661328-27.097792. EFFECT: increased fusion of protoplasts, increased quantity of regenerates. 3 tbl, 6 ex

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RU 2 138 552 C1

Authors

Davidenko E.A.

Avakjan Eh.R.

Aleshin N.E.

Aleshin E.P.

Dates

1999-09-27—Published

1997-11-11—Filed