FIELD: biotechnology, physiology, plant tissue cultures. SUBSTANCE: in medium for fusion of rice protoplasts dimethylsulfoxide is added additionally at the following ratio of components, weight %: CaCl2•2H2O 0.0478-0.0615; KH2PO4 0.054-0.0695; KNO3 0.0956-1.231; (NH4)2SO4 0.0427-0.0549; MgSO4•7H2O 0.0796-0.103; MnSO4•H2O 0.0032-0.0041; ZnSO4•7H2O 0.00064-0.00082; MnSO4•4H2O 0.0042-0.0054; H3BO3 0.0009-0.0012; KJ 0.00024-0.0003; CuSO4•5H2O 0.000007-0.000009; Na2MoO4•2H2O 0.00007-0.00009; CoCl2•6H2O 0.000007-0.0000009; Fe2SO4•7H2O 0.0089-0.0111; HO(CH2H4O)88H 34.97028- -40.705808; C6H14O6 22.310-28.716; NaOH 6.374-8.205; NH2CH2COOH 6.374- -7.705; C2H6SO 15.661328-27.097792. EFFECT: increased fusion of protoplasts, increased quantity of regenerates. 3 tbl, 6 ex
Authors
Dates
1999-09-27—Published
1997-11-11—Filed