METHOD FOR OBTAINING A CALLUS CULTURE OF YELLOW (GLAUCIUM FLAVUM GRANTZ) IN VITRO Russian patent published in 2023 - IPC C12N15/00 

FIELD: biotechnology.

SUBSTANCE: invention is a method for obtaining a callus culture of yellow goat (Glaucium flavum Grantz) in vitro, including sterilization of yellow goat seeds with a solution of antibacterial soap while stirring with a magnetic stirrer for 10–15 minutes, rinsing three times in sterile distilled water, placing sterile seeds on a solid nutrient medium without hormones of the following composition, in mg/l: water — 1,000 ml, agar-agar — 6500, then the seedlings are cultivated on a nutrient medium of the following composition, mg/l: water — 1,000 ml, NH₄NO₃ — 825, KNO₃ — 950, CaCl₂ x 2H₂O — 220, MgSO₄ x 7H₂O — 185, KH₂PO₄ — 85, KI — 0.415, H₃BO₃ — 3.1, MnSO₄ x 4H₂O — 11,15, ZnSO₄ x 7H₂O — 4.3, Na₂MoO₄ x 2H₂O — 0.125, CuSO₄ x 5H₂O — 0.0125, CoCl₂ x 6H₂O — 0.0125, FeSO₄ x 7H₂O — 13.9, Na-EDTA — 18.65, mesoinositol — 100, thiamine — 0.5, pyridoxine — 0.5, niacin — 0.5, sucrose — 15,000, agar — 6,500, at a temperature of 24± 1°C with a photoperiod of 16/8 hours (light/dark) to the third to fifth true leaves for three weeks until leaf explants are obtained, which are cut into small pieces and placed in a nutrient medium of the following composition, mg/l: water — 1,000 ml, NH₄NO₃ — 1,650, KNO₃ — 1900, CaCl₂ x 2H₂O — 440, MgSO₄ x 7H₂O — 370, KH₂PO₄ — 170, KI — 0.83, H₃BO₃ — 6.2, MnSO₄ x 4H₂O — 22.3, ZnSO₄ x 7H₂O — 8.6, Na₂MoO₄ x 2H₂O — 0.25, CuSO₄ x 5H₂O — 0.025, CoCl₂ x 6H₂O — 0.025, FeSO₄ x 7H₂O — 27.8, Na-EDTA (trilon B) — 37.3, mesoinositol — 100, thiamine — 0.5, pyridoxine — 0.5, nicotinic acid — 0.5, sucrose — 30,000, NUK — 1, BAP — 0.5, agar — 6,500; cultivation of callus culture is carried out in the dark, at a temperature of 24±1°C, chamber humidity of 70±5%, subculture cycle is 5 weeks. The invention makes it possible to obtain a callus culture of yellow (Glaucium flavum Grantz).

EFFECT: method has been developed for obtaining yellow callus cultures from seedling explants with the help of a climatostat.

1 cl, 5 dwg