NUTRIENT MEDIUM FOR CULTIVATION OF CALLUS CULTURE OF SPOTTED HEMLOCK (Conium maculatum L) Russian patent published in 2016 - IPC C12N5/04 A01H5/00 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology. Invention represents a nutrient medium for cultivation of callus tissue of spotted hemlock, containing components in the following amount, mg/l: KNO₃ 1900; KH₂PO₄ 170; NH₄NO₃ 1650; MgSO₄×7H₂O 370; CaCl₂×2H₂O 440; FeSO₄×7H₂O 37.3; Na₂EDTA×2H₂O 27.8; KI 0.83; H₃BO₃ 6.2; MnSO₄×4H₂O 22.3; CoCl₂×6H₂O 0.025; CuSO₄×5H₂O 0.025; ZnSO₄×7H₂O 8.6; Na₂MoO₄×2H₂O 0.25; thiamine-HCl 0.5-1.5; pyridoxine-HCl 0.4-0.6; nicotinic acid 0.4-0.6; sucrose 30,000; agar-agar 10,000; NUC 1-3; 6-BAP 0.1-1; distilled water - the rest.

EFFECT: invention makes it possible to cultivate callus tissue, as well as to receive high values of biomass gain of Conium maculatum.

1 cl, 2 tbl, 3 ex