METHOD OF PREPARING PREPARATION FOR DIRECTED DELIVERY OF ANTITUMOR DRUG IN CANCER CELL Russian patent published in 1999 - IPC

FIELD: medicine, oncology, protein chemistry. SUBSTANCE: retroplacental or abortive serum is tested, frozen and frozen out before onset of alpha-fetoprotein isolation followed by centrifugation. Then ammonium sulfate saturated solution is added to supernatant up to 30% of saturation, centrifuged again, crystalline ammonium sulfate is added to supernatant up to 70% of saturation and centrifuged. Obtained precipitate is dissolved in 10 mM sodium phosphate solution, pH 7.3, dialyzed, centrifuged and purified by affinity, ion-exchange and gel-exclusive chromatography followed by concentrating the purified alpha-fetoprotein to 1.5-5.0 mg/ml. Then the conjugation of alpha-fetoprotein (concentration is 1.5-2.0 mg/ml) with 2,3,7,8-tetrachlorodibenzo-p-dioxine dissolved in dioxane is carried out followed by addition of mannitol up to the concentration 5%, lyophilic drying, hermetic package under inert gas atmosphere and storage at -70 C. Invention can be used in chemotherapy of oncological patients. EFFECT: enhanced effectiveness of suppression of cancer cells proliferation of hormone-dependent organs and tissues. 1 cl, 1 ex