ANTITUMOR PEPTIDE PREPARATION BASED ON ALPHA-FETOPROTEIN FRAGMENT, ITS CONJUGATE, PHARMACEUTICAL COMPOSITION AND METHOD FOR TREATMENT OF HORMONE-DEPENDENT TUMORS Russian patent published in 2006 - IPC A61K38/12 A61P35/00 

FIELD: medicine, biotechnology, pharmacy.

SUBSTANCE: invention proposes an antitumor peptide preparation representing human alpha-fetoprotein (AFP) fragment with amino acid sequence given in the description (fig. 1) and comprising 243 amino acid residues in primary structure and molecular mass 27 kDa. This AFP fragment is able for binding with AFP receptors, to inhibit estradiol-induced growth of hormone-dependent tumors, and to perform vector function of molecule in delivery of cytotoxic preparation into tumor cells. The preparation is obtained by culturing cells E. coli transformed with plasmid vector comprising cDNA gene of the third domain of human AFP. The peptide product is modified by additional methionine residue (Met) at N-end and by two amino acid residues (Leu-Glu) at C-end. Also, invention relates to the peptide preparation conjugate and cytotoxic agent, pharmaceutical composition and a method of treatment. As a cytotoxic agent the conjugate comprises a substance chosen from the following group: paclitaxel, docetaxel, doxorubicin, vinblastin, diphtherin, ricin and others. Method of treatment involves administration in the patient the conjugate or composition in the effective amount. Invention provides enhancing effectiveness and selectivity in treatment of hormone-dependent tumors.

EFFECT: enhanced effectiveness of treatment, valuable medicinal properties of preparation and pharmaceutical composition.

7 cl, 7 dwg, 7 ex