METHOD OF PREPARING TRYPSIN STERILE SOLUTION FOR CULTURED CELLS Russian patent published in 1999 - IPC

FIELD: biotechnology. SUBSTANCE: invention relates to a method of preparing the trypsin sterile solution used for preparing primary and maintenance of diploid and transplanted cell strains. Method of preparing the trypsin sterile solution involves stages of preparing a mixture from dried inorganic salts, salts and trypsin mixture packaging, their sterilizing by accelerated electrons and dissolving before using the sterile mixture of salts and trypsin in sterile purified water directly. Proposed method of preparing provides the preparing trypsin sterile solution that exhibits high disaggregating capability during all period of the storage - 2 years at temperature (6 ± 4) C. EFFECT: improved method of preparing. 4 cl, 6 tbl, 4 ex