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2 084 523

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C1

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IPC

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Application

93047591/13, 1993-10-06

(22)

Actual filing date

1993-10-06

(45)

Published

1997-07-20

(72)

Inventor

Frolova I.V.Mazurkova N.A.Troshkova G.P.Kamshij L.P.Ginzburg I.A.

(73)

Holder

Gosudarstvennyj Nauchnyj Tsentr Virusologii I Biotekhnologii

METHOD OF PREPARING THE NUTRIENT MEDIUM FOR HUMAN BLOOD LYMPHOCYTES CULTURING Russian patent published in 1997 - IPC

Abstract RU 2084523 C1

FIELD: biotechnology. SUBSTANCE: nutrient medium has medium RPMI-1640 with HEPES-buffer, cattle serum blood, 3% L-glutamine solution with antibiotics and phytohemagglutinin. The obtained dissolved mixture is sterilized by filtration through diaphragms (pore diameter is 0.22 mcm), dosed in small bottle and subjected for lyophilic drying. Invention provides culturing the human blood lymphocytes at mitotic index = 30, not less, preparing the medium that is stable for prolonged period. Medium is used for diagnostic studies of chromosomal anomalies. EFFECT: improved method of preparing, enhanced quality of medium, simplified using. 2 cl

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RU 2 084 523 C1

Authors

Frolova I.V.

Mazurkova N.A.

Troshkova G.P.

Kamshij L.P.

Ginzburg I.A.

Dates

1997-07-20—Published

1993-10-06—Filed

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