Show metadata Hide metadata

(19)

(11)

2 143 002

(13)

(51)

IPC

(21) (22)

Application

97120833/13, 1997-12-24

(22)

Actual filing date

1997-12-24

(45)

Published

1999-12-20

(72)

Inventor

Gusjatiner M.M.Kaluzhskij V.E.Jampol'Skaja T.A.Plokhov A.Ju.Kopelevich V.M.Shul'Ga A.A.Sukhareva B.S.Darij E.L.Reznik A.I.Leonova T.V.

(73)

Holder

Aktsionernoe Obshchestvo Otkrytogo Tipa

METHOD OF γ-AMINOBUTYRIC ACID PRODUCING Russian patent published in 1999 - IPC

Abstract RU 2143002 C1

FIELD: amino acids, biotechnology, microbiology. SUBSTANCE: invention proposes a method of producing γ-aminobutyric acid (GABA) by metabolism of L-glutamic acid or its salts in the presence of biomass of bacterium Escherichia coli VKPM B-7460 or Escherichia coli VKPM B-7452 followed by purification and isolation of the end product. Decarboxylase activity of cells is 5 mcM GABA/min/mg of crude biomass and specific consumption of cells in the process of metabolism is 40 mg of crude biomass/1 g of GABA. The yield of the end product from glutamic acid is 55.6% wt.-% and that from sodium glutamate - 40 wt.-%. The content of GABA in the dry preparation - 98.7%. EFFECT: improved method of producing, increased yield and content. 1 tbl, 2 ex

Similar patents RU2143002C1

Title	Year	Author	Number
METHOD FOR PRODUCTION OF L-AMINO ACIDS USING BACTERIUM OF GENUS ESCHERICHIA CONTAINING NON-ACTIVE gadB GENE	2003	Fomina S.A. Voroshilova Eh.B. Skorokhodova A.Ju. Lunts M.G.	RU2264457C2
RECOMBINANT PLASMID DNA PSS5 ENCODING SYNTHESIS OF RECOMBINANT HUMAN ALPHA-2B INTERFERON, STRAIN OF ESCHERICHIA COLI SS5 AS PRODUCER OF RECOMBINANT HUMAN ALPHA-2B INTERFERON AND METHOD OF ALPHA-2B INTERFERON PREPARING	1999	Cherepanov P.A. Mikhajlova T.G. Cherepanov P.P. Martinenko Dmitrij Leonidovich Shevchuk Aleksandr Anatol'Evich Fedjukin V.S. Nikolaev T.M. Tolkachev B.B. Sventitskij E.N. Urakov N.N. Kalinin Ju.T. Denisov L.A. Tjagotin Ju.V. Martjushin S.V. Ishchenko A.M. Trofimov A.V. Poljakova E.A. Batarin V.I. Shalaeva O.N. Lisitskaja V.I.	RU2165455C1
RECOMBINANT PLASMID DNA PUABC 22 ENCODING MODIFIED ACTIVATOR OF URICASE TYPE PLASMINOGEN, NONTRANSLATED DNA-ELEMENT - ARTIFICIAL INTERGENE SEQUENCE MGP-14 AND STRAIN OF BACTERIUM ESCHERICHIA COLI - PRODUCER OF MODIFIED ACTIVATOR OF URICASE TYPE PLASMINOGEN	1999	Bibilashvili R.Sh. Belogurov A.A. Del'Ver E.P. Gurskij Ja.G. Minashkin M.M. Chigaeva S.M. Grigor'Eva N.V. Logunova N.A. Mjalkin A.A.	RU2140453C1
ESCHERICHIA COLI DNA FRAGMENT DETERMINING ENHANCED PRODUCTION OF L-AMINO ACIDS (VARIANTS) AND METHOD OF L-AMINO ACID PRODUCING	1999	Livshits V.A. Zakataeva N.P. Nakanishi Kazuo Aleshin V.V. Troshin P.V. Tokmakova I.L.	RU2175351C2
RECOMBINANT PLASMID DNA PBTN 11 DETERMINING SYNTHESIS OF CRYSTALLINE INSECTICIDE DELTA-ENDOTOXIN CRY IIIA-TYPE, METHOD OF ITS CONSTRUCTION AND STRAIN OF BACTERIUM PSEUDOMONAS PUTIDA CONTAINING RECOMBINANT PLASMID DNA PBTN 11 - A PRODUCER OF CRYSTALLINE INSECTICIDE DELTA-ENDOTOXIN CRY IIIA-TYPE SHOWING ACTIVITY AGAINST INSECTS OF COLEOPTERA ORDER	1995	Dobritsa A.P. Koretskaja N.G. Kochetkov V.V. Svetoch O.E. Loseva O.I.	RU2103363C1
DNA FRAGMENT RHTB ENCODING SYNTHESIS OF PROTEIN RHTB THAT DETERMINES RESISTANCE OF BACTERIUM ESCHERICHIA COLI TO L-HOMOSERINE AND METHOD OF L-AMINO ACID PRODUCING	1998	Livshits V.A. Zakataeva N.P. Aleshin V.V. Belareva A.V. Tokmakova I.L.	RU2144564C1
RECOMBINANT PLASMID DNA PPINS07 ENCODING FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN AND STRAIN OF BACTERIUM ESCHERICHIA COLI - PRODUCER OF FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN	1999	Korobko V.G. Boldyreva E.F. Shingarova L.N. Vul'Fson A.N. Kostromina T.I. Miroshnikov A.I. Gavrikov V.G. Kalinin Ju.T. Stepanov A.V.	RU2144957C1
METHOD FOR PRODUCING L-AMINO ACIDS WITH USE OF BACTERIA OF ENTEROBACTERIACEAE FAMILY	2010	Savrasova Ekaterina Alekseevna Stojnova Natalija Viktorovna Nonaka Gen Jamazaki Sinsuke Takumi Kazukhiro	RU2460793C2
RECOMBINANT PLASMID DNA PPINS16 ENCODING FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN, RECOMBINANT PLASMID DNA PPINS25 ENCODING FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN AND STRAIN OF BACTERIUM ESCHERICHIA COLI - PRODUCER OF FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN	1999	Korobko V.G. Boldyreva E.F. Shingarova L.N. Miroshnikov A.I. Gavrikov V.G. Stepanov A.V. Kalinin Ju.T.	RU2143493C1
METHOD OF PRODUCING L-CYSTEINE, L-CYSTINE, S-SULPHOCYSTEINE OR L-CYSTEINE THIAZOLIDINE DERIVATIVE, OR MIXTURE THEREOF USING BACTERIA OF ENTEROBACTERIACEAE FAMILY	2010	Zijatdinov Mikhail Kharisovich Samsonov Viktor Vasil'Evich Gusjatiner Mikhail Markovich	RU2458982C2

RU 2 143 002 C1

Authors

Gusjatiner M.M.

Kaluzhskij V.E.

Jampol'Skaja T.A.

Plokhov A.Ju.

Kopelevich V.M.

Shul'Ga A.A.

Sukhareva B.S.

Darij E.L.

Reznik A.I.

Leonova T.V.

Dates

1999-12-20—Published

1997-12-24—Filed