DNA RHTC FRAGMENT ENCODING SYNTHESIS OF RHTC PROTEIN THAT DETERMINES ENHANCED RESISTANCE OF BACTERIUM ESCHERICHIA COLI TO L-THREONINE AND METHOD OF L-AMINO ACID PRODUCING Russian patent published in 2000 - IPC

Abstract RU 2148642 C1

FIELD: molecular biology, biotechnology, genetic engineering. SUBSTANCE: DNA fragment rhtC encodes synthesis of RhtC protein that determines enhanced resistance of bacterial cells Escherichia coli to L-threonine. Strains of Escherichia coli that are able to enhanced production of L-amino acids as compared with plasmid pRhtC-free strains are constructed on the basis of multicopy plasmid pRhtC. Amino acids are obtained by culturing corresponding strains in suitable nutrient media followed by isolation and purification of the end amino acid. EFFECT: increased yield of L-amino acids. 6 cl, 7 dwg, 6 tbl, 5 ex

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RU 2 148 642 C1

Authors

Livshits V.A.

Zakataeva N.P.

Aleshin V.V.

Belareva A.V.

Tokmakova I.L.

Dates

2000-05-10—Published

1998-12-23—Filed