FIELD: medicine. SUBSTANCE: method involves preparing patient blood samples to be studied, preparing reagents by dissolving iron sulfate, luminol, phosphate buffer with pH equal to 7.5, preparing lipoproteid model, mixing the samples and the reagents. Chemiluminescence peak amplitudes are recorded in the produced mixture, their difference being used for making conclusions concerning total antioxidation blood activity. Citrated blood plasma containing no blood platelets is used as blood fraction under study. Iron sulfate solution is prepared at 0.01 mM concentration by dissolving the weighed portion in boiling water. The solution is centrifuged in hermetically sealed reservoir with following transfer of the supernatant into the other test tube and placing the supernatant under the chemically inert oil layer. Luminol solution is prepared by diluting the basic (maternal) solution with distilled water in 1:1000 proportion, 2% hydrogen peroxide solution is used as a substance initiating reaction. The medium under study is united with the reagents in the following order. Lipoproteid model, citrated patient blood plasma containing no blood platelets are introduced into phosphate buffer and then iron sulfate, luminol solutions are added and hydrogen peroxide solution is introduced into the reaction later after switching in luminescence signal recording. The examination is carried out in stirring the mixture beginning from the moment the lipoproteid model is introduced. After adding hydrogen peroxide solution, metering unit is not removed from measuring chamber socket until the examination is over. EFFECT: high accuracy of measurements. 5 cl
