FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology. A method is described for constructing a library of polynucleotides of interest in a poxvirus-based vector system, such as the smallpox vaccine virus, where the polynucleotides of interest encode the polypeptides of interest. The method involves constructing a library in the presence of a poxvirus assembly inhibitor, such as rifampicin, which allows the construction of libraries with greater complexity and diversity than in previous methods.
EFFECT: invention expands the arsenal of methods for constructing libraries of polynucleotides.
32 cl, 1 tbl, 1 ex
| Title |
Year |
Author |
Number |
| DISPLAY OF AN INTEGRAL MEMBRANE PROTEIN ON EXTRACELLULAR ENVELOPED POXVIRUS VIRIONS |
2017 |
- Smith, Ernest S.
- Paris, Mark
- Scrivens, Maria G. M.
- Kirk, Renee A.
- Cornelison, Angelica A.
|
RU2759846C2 |
| VIRAL VACCINES |
2017 |
|
RU2778312C2 |
| VIRAL VECTOR MANUFACTURE |
2014 |
|
RU2658487C2 |
| LIBRARY OF CONCENTRATION-DEPENDENT ION BINDING MOLECULES |
2012 |
- Igawa, Tomoyuki
- Ishii, Shinya
- Funaki, Miho
- Hironiwa, Naoka
- Shimizu, Shun
|
RU2812861C1 |
| COMBINATION OF POXVIRUS ENCODING HUMAN PAPILLOMA VIRUS POLYPEPTIDES, AND IL-2 WITH ANTI-PD-L1 ANTIBODY |
2020 |
- Bendjama Kaidre
- Brandely Talbot Maud
- Tavernaro Annette
|
RU2824962C1 |
| CANCER THERAPY BY COMBINED USE OF ONCOLYTIC SMALLPOX VACCINE VIRUS AND IMMUNE CONTROL POINT INHIBITOR, AND PHARMACEUTICAL COMPOSITION AND COMBINED DRUG FOR USE IN CANCER THERAPY |
2019 |
- Nakao Shinsuke
- Kawase Tatsuya
|
RU2788874C2 |
| M2-DEFECTIVE POXVIRUS |
2019 |
- Klyajnpeter, Patritsia
- Marshan, Zhan-Batist
- Remi, Kristell
- Shmitt, Doris
|
RU2819245C2 |
| COMPOSITIONS OF RECOMBINANT CDV AND ITS USE |
2011 |
- Odonne Zhan-Kristof
- Minke Zhjul'
|
RU2567337C2 |
| COMBINATION THERAPY FOR TREATING CANCER WITH POXVIRUS EXPRESSING TUMOUR ANTIGEN AND ANTAGONIST AND/OR AGONIST OF IMMUNE CHECKPOINT INHIBITOR |
2014 |
- Rauntri, Rajan
- Foj, Syuzan
- Mendl, Stefani
- Delker, Alen
|
RU2714142C2 |
| ONCOLYTIC VIRUS FOR IMMUNOLOGIC CONTROL POINT MODULATORS EXPRESSION |
2015 |
- Silvestr Natali
- Gejst Mishel
- Rittner Karola
- Marshan Zhan-Batist
- Tiudelle Kristin
|
RU2696312C2 |
