FIELD: biotechnology.
SUBSTANCE: method for obtaining a polynucleotide library is presented. A polynucleotide library containing a plurality of barcoded polynucleotides obtained by this method is also presented. The library used to sequence one or more of a variety of target polynucleotides. In addition, the invention relates to a method for sequencing and methods for analyzing a transcriptome.
EFFECT: performing transcriptomic sequencing, such as RNA sequencing, obtaining a picture of the transcriptome of individual cells in combination with full-length immune receptor sequences so that information about the immune repertoire and the transcriptome of the cell can be obtained.
137 cl, 5 dwg, 5 tab, 8 ex
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