FIELD: biochemistry, phytopathology. SUBSTANCE: invention relates to assay of fungal pathogens using specific primers in polymerization chain reaction. Method of detection of phytopathogenic fungus involves the following stages: isolation of DNA from plant leaf infected with pathogen, amplification of part of intron transcribing region of pathogen using isolated DNA as matrix and using polymerase chain reaction with specific primer and visualization of amplified part of intron transcribing region. Structure of some DNA molecules encoding intron transcribing sequence using for construction of oligonucleotide primers for detection of phytopathogenic fungus is determined. Primers for assay of phytopathogenic fungi are constructed. Quantitative colorimetric analysis for detection of phytopathogenic fungus is developed. Method ensures to detect phytopathogenic fungi, for example, Microdochium nivate, Septoria nodorum, Septoria tritici, Pseudocercosporella herpotrichoides, Mycosphaerella fijiensis, Mycosphaerella musicola, Fusarium culmorum, Fusarium graminearum, Fusarium moniliform. EFFECT: improved method of assay. 18 cl, 5 tbl, 13 ex
| Title | Year | Author | Number |
|---|---|---|---|
| DIAGNOSTIC BASIS FOR DETECTION OF MUTATION OF FUNGAL CYTOCHROME B | 2000 |
|
RU2244750C2 |
| METHOD FOR IDENTIFYING PHYTOPATHOGENIC FUNGI ZYMOSEPTORIA TRITICI AND PARASTAGONOSPORA NODORUM BY PCR | 2023 |
|
RU2824044C1 |
| FUNGICIDE AGENT FOR PLANT PROTECTION | 1994 |
|
RU2121792C1 |
| STRUCTURE OF DNA, STRUCTURE OF RECOMBINANT DNA AND METHOD OF PLANT PRODUCING | 1994 |
|
RU2136757C1 |
| FUNGAL ENDOPHYTES EPICHLOE / NEOTYPHODIUM SPP. FOR ARTIFICIAL INFECTION OF SECALE SPP. AND USE THEREOF | 2014 |
|
RU2683379C2 |
| METHOD OF CONTROLLING RESISTANT PATHOGENIC FUNGI QoI | 2009 |
|
RU2527029C2 |
| METHOD OF DETERMINING STABILITY OF FUNGAL PHYTOPATHOGEN, ALLELE-SPECIFIC PRIMER, AND COLLECTION | 2002 |
|
RU2296162C2 |
| MYCOSPHAERELLA SP. STRAIN, COMPOSITIONS AND METHODS FOR FUSARIOSIS CONTROL | 2012 |
|
RU2705286C2 |
| MICROBACTERIUM STRAIN, COMPOSITIONS AND METHODS TO COMBAT FUSARIOSIS | 2012 |
|
RU2634386C2 |
| COMPOSITIONS AND METHODS FOR FUSARIUM DISEASE CONTROL | 2019 |
|
RU2736382C1 |
