METHOD FOR DETERMINING ENDOTOXIN ACTIVITY Russian patent published in 2001 - IPC

FIELD: medicine. SUBSTANCE: method involves mixing Limulus polyphemus lisate and the diluted sample under test, with polymer causing coagulum production detected in the last dilution. The polymer causing coagulum production is identified by examining protein fractals structure after drying the mixture. Mixing Limulus lisate and the diluted sample under test is carried out by placing 2-8 mcl of Limulus lisate solution and the diluted sample under test onto plastic surface. The plastic surface with reagents placed thereon is covered with cover and incubated at 37 C during 30 min. It is uncovered and kept in the thermostat until reagent mixture drops dry. The polymer causing coagulum production is recognized by detecting structure of protein fractals produced. Endotoxin being available, the fractals as specific crystal-shaped structures are observed. Otherwise, when no endotoxin being found or negative control being the case, smooth field with rare intrusions of rhombic salt crystals is observed in the peripheral part of the field. EFFECT: enhanced sensitivity in determining endotoxin activity. 4 cl, 4 dwg, 1 tbl