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2 447 446

(13)

(51)

IPC

G01N33/53(2006-01-01)

G01N33/573(2006-01-01)

C12N9/50(2006-01-01)

(21) (22)

Application

2010127036/10, 2010-07-01

(24)

Start date

2010-07-01

(22)

Actual filing date

2010-07-01

(45)

Published

2012-04-10

(72)

Inventor

Zinkevich Oleg DanilovichSafina Nehllja AkhmetovnaBarysheva Natalija Olegovna

(73)

Holder

Gosudarstvennoe Obrazovatel'Noe Uchrezhdenie Dopolnitel'Nogo Professional'Nogo Obrazovanija Gosudarstvennaja Meditsinskaja Akademija Federal'Nogo Agentstva Po Zdravookhraneniju I Sotsial'Nomu Razvitiju"

METHOD FOR MEASURING ACTIVITY OF PROTEOLYTIC ENZYMES OF VARIOUS TYPES OF ACTIVE CENTRES ABLE TO SPLIT IMMUNOGLOBULINS IgA, IgM, IgG Russian patent published in 2012 - IPC G01N33/53 G01N33/573 C12N9/50

Abstract RU 2447446 C2

FIELD: medicine.

SUBSTANCE: method involves tray coating with a bacterial antigen of any representative of an opportunistic microflora, coating of a sorbed antigen with IgG, IgM and IgA antibodies, a proteolytic reaction by incubation in tray wells of an analysed biological fluid together with inhibitors of active centres of the enzymes (serine, cysteine, metal-dependent), washing of the reaction products and calculation of enzymatic activity with appropriate type of the active centre.

EFFECT: invention provides simultaneous measurement of activity of proteinase of various origin and of various types of the active centres.

4 tbl, 4 ex

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RU 2 447 446 C2

Authors

Zinkevich Oleg Danilovich

Safina Nehllja Akhmetovna

Barysheva Natalija Olegovna

Dates

2012-04-10—Published

2010-07-01—Filed