IMMUNOASSAY OF SOLUBLE FIBRIN POLYMERS Russian patent published in 2001 - IPC

FIELD: immunology. SUBSTANCE: invention relates to method of determination, detection or quantitation in vitro of soluble cross-linked fibrin polymers Des AABB and cross-not-linked fibrin polymers Des AABB in sample obtained from mammal. Method involves contact of indicated sample with antibody or its immunoreactive fragments. Fragments do not bind specifically with: a) fibrinogen; b) fibrinogen catabolites; c) fibrin monomers Des AA, d; d) fibrin polymers Des AA; e) fibrin monomers Des AABB; f) cross-linked fibrinogen; g) complex fibrin monomer Des AA/fibrinogen and h) fibrin catabolites. Methods of estimation of predisposition to thrombotic state, confirmation of diagnosis of the presence of thrombotic state and monitoring of thrombotic state in mammal involve determination of amount of soluble cross-linked fibrin polymers Des AABB and soluble cross-not-linked fibrin polymers Des AABB in sample obtained from mammal. Invention proposes kits used for determination, detection or quantitation of soluble cross-linked fibrin polymers Des AABB and soluble cross-not-linked fibrin polymers Des AABB in sample, for estimation of predisposition to thrombotic state in mammal, for confirmation of diagnosis of thrombotic state and for determination of stage of thrombotic state in mammal. Invention provides to determine predisposition to thrombotic state in mammal reliably. EFFECT: improved method of immunoassay. 34 cl, 12 tbl