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2 175 552

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IPC

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Application

2000106473/14, 2000-03-16

(24)

Start date

2000-03-16

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Actual filing date

2000-03-16

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Published

2001-11-10

(72)

Inventor

Chirjat'Ev E.A.Kalinin E.P.Rusakova O.A.

(73)

Holder

Tjumenskaja Gosudarstvennaja Meditsinskaja Akademija

METHOD OF PREPARING ANTICOAGULANT FROM SAPROPEL Russian patent published in 2001 - IPC

Abstract RU 2175552 C1

FIELD: medicine, biochemistry. SUBSTANCE: extract is prepared from sapropel by three-fold volume of 0.1 M ammonium solution and extract is kept at -15 C for 24 h. After defrosting at the room temperature supernatant is decanted and concentrated by evaporation under vacuum. The concentrated fraction is centrifuged and dialyzed. Nondyalizable fraction is treated with ammonium sulfate at 100% degree of saturation and the formed precipitate is discarded. The purified fraction is desalted with 96% ethyl alcohol followed by gel-filtration on column with Sephadex G-50. Eluates are combined and dried. EFFECT: enhanced purity of end product. 1 tbl

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RU 2 175 552 C1

Authors

Chirjat'Ev E.A.

Kalinin E.P.

Rusakova O.A.

Dates

2001-11-10—Published

2000-03-16—Filed

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