METHOD OF IMMOBILIZATION OF OLIGONUCLEOTIDES CONTAINING UNSATURATED GROUPS IN POLYMERIC HYDROGELS IN FORMING MICROCHIP Russian patent published in 2001 - IPC

FIELD: biochemistry. SUBSTANCE: described is method of immobilization of oligonucleotides in polymeric hydrogels including polyacrylamide gel. Said method comprises copolymerizing oligonucleotides modified by unsaturated groups of general formula I: with unsaturated monomers which represent basic constituents of the resulting hydrogel. Also described are modified oligonucleotides of formula I wherein R¹, R², R³ are H, C₁-C₆ alkyl, Ph, PhCH_2-; Z is (CH₂)_nCH(CH₂OH)CH₂OX wherein n is 1-6; or (CH₂)_n-OX, wherein n is 2-6; and X is phosphodiether group binding unsaturated fragment with 5-5'and/or 3'-oligonucleotide end; R⁴ is H, (CH₂)_n-OH, wherein m is 2-6; Y is p-C₆H₄)_n wherein n is 0-2. Oligonucleotides are modified by phosphoamidite of general formula II: wherein R¹, R² and Y are as defined in formula I, R³ is C₁-C₆ alkyl, R⁴ is H, (CH₂)_n-ОДМТ, wherein n is 2-6, or by acylation of oligonucleotide containing aminolinic by activated unsaturated acid ester. EFFECT: high degree of immobilization of oligonucleotides in polymeric hydrogel in forming microchip. 19 cl, 5 dwg, 10 ex, 2 tbl