METHOD FOR POLYMERIZATION IMMOBILIZATION OF BIOLOGICAL MACROMOLECULES AND COMPOSITION FOR ITS REALIZATION Russian patent published in 2003 - IPC

FIELD: polymers, biochemistry. SUBSTANCE: invention relates to composition of the composite: K = A^a+B^b+C^c+D^d+E^e+F^f wherein K is composition; A is acrylamide, methacrylamide, N-[tris-(hydroxymethyl)-methyl]acrylamide, 2-hydroxyethylmethacrylate, methylmethacrylate or other polymer based on derivatives of acrylic, methacrylic, cinnamic, crotonic, vinylbenzoic or other unsaturated acids; B is N,N'-methylene-bis-acrylamide, N,N'-(1,2- dihydroxyethylene)-bis-acrylamide, polyethylene glycolacrylate, their mixture or other symmetric or non- symmetric cross-linking agent based on derivatives of acrylic, methaacrylic, cinnamic, crotonic, vinylbenzoic or other unsaturated acids; C is oligonucleotide, nucleic acid or other molecule carrying active group, among them amino- or sulfhydryl group; D means components of medium in carrying out of polymerization immobilization, namely, glycerol, sucrose, polyalcohols; E is water, N,N- dimethylformamide, dimethylsulfoxide and other polar and nonpolar solvents; F is ammonium persulfate, potassium persulfate, methylene blue, fluorescein, N,N',N'-tetramethylethylenediamine, hydrogen peroxide, 4-(N,N- -dimethylamino)-pyridine, triethylamine, acetone or any other initiating agent for chemical or photoinitiation of polymerization reaction; a, b, c, d, e, f are percent content (X) of each component in the composition (X = m/v x 100% for solid substances or X = v/v x 100% for liquid substances), 3 <a + b <40%; 0 <c <10%; 0 <d <95%; 0 <e <95%; 0 <f <90%; for polymerization immobilization of different molecules as component of linear or three-dimensional porous polymer, among them oligonucleotides, proteins and nucleic acids containing in structure active groups, among them aliphatic amino- and/or sulfhydryl groups in addition or substitution reactions (radical, nucleophilic, electrophilic and so on) in the process of synthesis of polymer or in photo- and chemical initiation of polymerization; to method for polymerization of molecules, among them oligonucleotides, proteins and nucleic acids comprising in structure active groups, in composition of linear or three-dimensional porous polymer preparing in the base of composition (K). EFFECT: improved immobilization method. 22 cl, 3 dwg, 3 ex