ISOLATED DNA MOLECULE ENCODING GLUCAGON RECEPTOR, DNA-CONSTRUCTION, CELL LINE, METHOD OF PREPARING GLUCAGON RECEPTOR, ISOLATED GLUCAGON RECEPTOR PEPTIDE, ISOLATED ANTIBODY, PROBE, METHOD OF DETECTION OF GLUCAGON ANTAGONIST PRESENCE Russian patent published in 2002 - IPC

FIELD: molecular biology, medicine, endocrinology. SUBSTANCE: invention relates to glucagon receptors. Invention proposes the isolated DNA molecule encoding glucagon receptors or glucagon peptide receptors. DNA-construction provides expression of isolated DNA molecule and its insertion into cells of lines COS or BHK. Invention proposes also a method of preparing glucagon receptors involving culturing cell lines COS or BHK and isolation of the end product. Invention presents also a monoclonal antibody binding specifically with glucagon receptors and blocking glucagon binding with glucagon receptors. Invention relates to also a method of detection of glucagon antagonist involving agonist binding with isolated glucagon receptors. Invention can be associated with stimulation of glycolysis and glycogenolysis and study of glucagon role in diabetes mellitus etiology. EFFECT: improved method of preparing DNA, glucagon and peptide receptors. 13 cl, 5 dwg, 8 tbl