POLYNUCLEOTIDE ANTITUBERCULOSIS VACCINE Russian patent published in 2002 - IPC

FIELD: medicine, phthisiology, protein engineering, vaccines, pharmacy. SUBSTANCE: invention proposes a polypeptide comprising the transcription promoter CMV bound with the first cistron. The first cistron comprises the transcription promoter CMV and presents gene encoding mycobacterial protein 85A or B or C. Indicated gene is bound by a control sequence being the latter comprises terminator and promoter transcription BGH or JRES. The control sequence can be bound with the second cistron presenting gene encoding GM-CSF, IL-12, interferon or a member of family B7 of T-cellular bone-stimulating proteins. Indicated gene can be bound with transcription terminator BGH, gene determining the resistance to ampicillin or kanamycin bound with site involving the point of replication start. Method of induction of antimycobacterial immune responses and immune responses against epitope of mycobacterial protein 85A involves the administration of the above indicated polynucleotide in vertebrate. Vaccine comprises the effective dose the above indicated polynucleotide and a pharmaceutically acceptable carrier and provides the induction of immune response in vertebrate against mycobacterial protein 85A. Invention ensures to prepare immune sera against Mycobacterium tuberculosis antigens. EFFECT: vaccine against above, valuable medicinal properties. 6 cl, 21 dwg