SURFACE ANCHOR VECTOR (VERSIONS), STRAIN ESCHERICHIA COLI TRANSFORMED BY VECTOR (VERSIONS), METHOD OF EXPRESSION OF FOREIGN PROTEINS ON CELLULAR SURFACE Russian patent published in 2002 - IPC

FIELD: biotechnology, molecular biology, protein engineering. SUBSTANCE: surface anchor vector comprises segments of gene encoding protein that induces formation of ice microcrystals (INP) from Pseudomonas syringe and novel restriction sites for insertion foreign genes by C-end of INP. Gene segments comprise N-terminal secretion signal, C-terminal targeting signal and can involve central repeating domain. Gene encoding carboxymethylcellulase or levansucrase is cloned into vector, vector is transformed into Escherichia coli cells and transformed strain is obtained. Expression of protein on cellular surface is performed by the transformed strain culturing. Invention ensures to obtain foreign proteins on surface of cells. EFFECT: improved method of expression and proteins preparing. 14 cl, 12 dwg, 13 ex