FIELD: medicine.
SUBSTANCE: invention is related to mutant of rumen bacterium of MANNHEIMIA type, which is produced by inactivation of lactate dehydrogenase gene (ldhA) and pyruvate-formate lyase gene (pfl). Mutant of rumen bacteria of MANNHEIMIA type, which is produced by inactivation of lactate dehydrogenase gene (ldhA), pyruvate-formate lyase gene (pfl), phosphotrans acetylase gene (pta), and acetate kinase gene (ackA). Mutant of rumen bacteria of MANNHEIMIA type, produced by means of inactivation of lactate dehydrogenase gene (ldhA), gene of pyruvate-formate lyase (pfl) and gene of phosphoenolpyruvate carboxylase (ppc). To methods for production of succinic acid, by cultivation of above mentioned mutants in anaerobic conditions.
EFFECT: bacterial mutants of the present invention are able to produce succinic acid in high concentration, while production of other organic acids is reduced or practically unavailable.
28 cl, 13 dwg, 1 tbl, 8 ex
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