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IPC

(21) (22)

Application

99109022/13, 1997-10-01

(24)

Start date

1997-10-01

(22)

Actual filing date

1997-10-01

(45)

Published

2003-02-10

(72)

Inventor

Ankenbauer Val'TraudShmitts-Agkheguian GudrunBonch-Osmolovskaja ElizavetaSvetlichnyj VitalijMarkau UrsulaAngerer BernkhardRajser Astrid

(73)

Holder

Roshe Diagnostiks Gmbkh

METHOD OF CDNA SYNTHESIS USING THERMOSTABLE DNA-POLYMERASE Russian patent published in 2003 - IPC

Abstract RU 2198222 C2

FIELD: biochemistry, molecular biology, genetic engineering. SUBSTANCE: DNA synthesis is carried out using thermostable DNA-polymerase. DNA-polymerase is prepared from Anaerocellum thermophilum or Escherichia coli LE3922pUB520pAR10. DNA-polymerase exhibits 5'-3'-polymerase activity and reverse transcriptase activity but it does not show 3'-5'-exonuclease activity in the presence of magnesium ions and in the absence of manganese ions. Invention ensures to carry out cDNA synthesis at higher temperatures. EFFECT: valuable properties of DNA-polymerase. 3 cl, 5 dwg, 7 ex

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RU 2 198 222 C2

Authors

Ankenbauer Val'Traud

Shmitts-Agkheguian Gudrun

Bonch-Osmolovskaja Elizaveta

Svetlichnyj Vitalij

Markau Ursula

Angerer Bernkhard

Rajser Astrid

Dates

2003-02-10—Published

1997-10-01—Filed